Incubation:Article Title: Wear resistant antimicrobial compositions and methods of use
Article Snippet: Method Parameters: Test Substance Formula: Test composition C Raw Material Concentration Bardac DM50 (Alkyl benzyl quat 50% 50% active)) Vantocil P (PHMG 20% active) 50% Test Substance Dilutions: Prepared on 1-20-09: Test Solution (Volume of Desired Test Substance/ Concentration Diluent Total Volume) 1:64 410 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.65) Prepared on 1-20-09: Test Solution (Volume of Desired Test Substance/ Concentration Diluent Total Volume) 1:64 510 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.84) Test Systems: Staphylococcus aureus ATCC 6538 Klebsiella pneumoniae ATCC 4352 Staphylococcus aureus (MRSA) ATCC 33592 Enterococcus faecalis (VRE) ATCC 51299 Soil Challenge: 5% Bovine Serum Test Material: 1′′ × 1′′ Glass Surfaces 1 mm thick non-frosted microscope slides, cut into squares Test Substance 50 μL applied and spread end to end with a pipette tip Application: Number of Wears 12 Total (6 dry and 6 wet) Performed: Number of Re-inoculations 5 Total Performed: Neutralizer Medium: 30 mL Dey Engley Broth A neutralizer screen was performed as part of the testing, verified that the neutralizer adequately neutralized the product and was not detrimental to the tested organisms. .. Plating Medium: Tryptone Glucose Extract Agar Incubation: 35° C. for 48 hours Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL MRSA Initial 148 × 103 155 × 103 1.5 × 105 ATCC 33592 Reinoculation 61 × 103 45 × 103 5.3 × 104 Final 124 × 106 134 × 106 1.3 × 108 VRE Initial 34 × 104 35 × 104 3.5 × 105 ATCC 51299 Reinoculation 94 × 103 136 × 103 1.2 × 105 Final 50 × 107 35 × 107 4.2 × 108 MRSA ATCC 33592 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 253 × 102, 263 × 102 7.59 × 105, 7.89 × 105 7.8 × 105 N/A 268 × 102, 251 × 102 8.04 × 105, 7.53 × 105 Test 0 × 100, 0 × 100 <30, <30, <30 >99.996 Composition C 0 × 100, 1 × 100 <30, 3.00 × 101 *CFU/mL × 30 VRE ATCC 51299 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 67 × 103, 90 × 103 2.01 × 106, 2.70 × 106 2.2 × 106 N/A 67 × 103, 66 × 103 2.01 × 106, 1.98 × 106 Test 4 × 102, 4 × 102 1.20 × 104, 1.20 × 104 <6.0 × 103 >99.727 Composition C 0 × 100, 0 × 100 <30, <30 *CFU/mL × 30 Test Date: Jan. 22, 2009 Date Results Read: Jan. 26, 2009 Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL Staphylococcus aureus Initial 114 × 103 100 × 103 1.1 × 105 ATCC 6538 Reinoculation 43 × 103 55 × 103 4.9 × 104 Final 111 × 106 112 × 106 1.1 × 108 Klebsiella pneumoniae Initial 117 × 103 142 × 103 1.3 × 105 ATCC 4352 Reinoculation 55 × 103 60 × 103 5.8 × 104 Final 109 × 107 129 × 107 1.2 × 109 Staphylococcus aureus ATCC 6538 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 30 × 103, 19 × 103 9.00 × 105, 5.70 × 105 8.0 × 105 N/A 31 × 103, 26 × 103 9.30 × 105, 7.80 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.996 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Klebsiella pneumoniae ATCC 4352 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 22 × 103, 18 × 103 6.60 × 105, 5.40 × 105 3.9 × 105 N/A 48 × 102, 75 × 102 1.44 × 105, 2.25 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.992 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Conclusion: To be defined as a sanitizer, the test substances on the hard inanimate surface must reduce the total number of organisms by at least 99.9% on the surface within a 5 minute period (after the final inoculation). ..
Article Title: Wear resistant antimicrobial compositions and methods of use
Article Snippet: Method Parameters: Test Substance Formula: Test composition C Raw Material Concentration Bardac DM50 (Alkyl benzyl quat 50% active)) 50% Vantocil P (PHMG 20% active) 50% Test Substance Dilutions: Prepared on Jan. 20, 2009: Desired Test Solution (Volume of Test Concentration Diluent Substance/Total Volume) 1:64 410 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.65) 1:64 510 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.84) Test Systems: Staphylococcus aureus ATCC 6538 Klebsiella pneumoniae ATCC 4352 Staphylococcus aureus (MRSA) ATCC 33592 Enterococcus faecalis (VRE) ATCC 51299 Soil Challenge: 5% Bovine Serum Test Material: 1′′×1′′ Glass Surfaces 1 mm thick non-frosted microscope slides, cut into squares Test Substance Application: 50 μL applied and spread end to end with a pipette tip Number of Wears Performed: 12 Total (6 dry and 6 wet) Number of Re-inoculations Performed: 5 Total Neutralizer Medium: 30 mL Dey Engley Broth A neutralizer screen was performed as part of the testing, verified that the neutralizer adequately neutralized the product and was not detrimental to the tested organisms. .. Plating Medium: Tryptone Glucose Extract Agar Incubation: 35° C. for 48 hours Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL MRSA Initial 148 × 103 155 × 103 1.5 × 105 ATCC 33592 Reinoculation 61 × 103 45 × 103 5.3 × 104 Final 124 × 106 134 × 106 1.3 × 108 VRE Initial 34 × 104 35 × 104 3.5 × 105 ATCC 51299 Reinoculation 94 × 103 136 × 103 1.2 × 105 Final 50 × 107 35 × 107 4.2 × 108 MRSA ATCC 33592 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 253 × 102, 263 × 102 7.59 × 105, 7.89 × 105 7.8 × 105 N/A 268 × 102, 251 × 102 8.04 × 105, 7.53 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 >99.996 Composition C 0 × 100, 1 × 100 3.00 × 101 *CFU/mL × 30 VRE ATCC 51299 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 67 × 103, 90 × 103 2.01 × 106, 2.70 × 106 2.2 × 106 N/A 67 × 103, 66 × 103 2.01 × 106, 1.98 × 106 Test 4 × 102, 4 × 102 1.20 × 104, 1.20 × 104 <6.0 × 103 >99.727 Composition C 0 × 100, 0 × 100 <30, <30 *CFU/mL × 30 Test Date: Jan. 22, 2009 Date Results Read: Jan. 26, 2009 Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL Staphylococcus Initial 114 × 103 100 × 103 1.1 × 105 aureus Reinoculation 43 × 103 55 × 103 4.9 × 104 ATCC 6538 Final 111 × 106 112 × 106 1.1 × 108 Klebsiella Initial 117 × 103 142 × 103 1.3 × 105 pneumonias Reinoculation 55 × 103 60 × 103 5.8 × 104 ATCC 4352 Final 109 × 107 129 × 107 1.2 × 109 Staphylococcus aureus ATCC 6538 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 30 × 103, 19 × 103 9.00 × 105, 5.70 × 105 8.0 × 105 N/A 31 × 103, 26 × 103 9.30 × 105, 7.80 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.996 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Klebsiella pneumoniae ATCC 4352 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 22 × 103, 18 × 103 6.60 × 105, 5.40 × 105 3.9 × 105 N/A 48 × 102, 75 × 102 1.44 × 105, 2.25 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.992 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Conclusion: To be defined as a sanitizer, the test substances on the hard inanimate surface must reduce the total number of organisms by at least 99.9% on the surface within a 5 minute period (after the final inoculation). ..
Suspension:Article Title: Wear resistant antimicrobial compositions and methods of use
Article Snippet: Method Parameters: Test Substance Formula: Test composition C Raw Material Concentration Bardac DM50 (Alkyl benzyl quat 50% 50% active)) Vantocil P (PHMG 20% active) 50% Test Substance Dilutions: Prepared on 1-20-09: Test Solution (Volume of Desired Test Substance/ Concentration Diluent Total Volume) 1:64 410 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.65) Prepared on 1-20-09: Test Solution (Volume of Desired Test Substance/ Concentration Diluent Total Volume) 1:64 510 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.84) Test Systems: Staphylococcus aureus ATCC 6538 Klebsiella pneumoniae ATCC 4352 Staphylococcus aureus (MRSA) ATCC 33592 Enterococcus faecalis (VRE) ATCC 51299 Soil Challenge: 5% Bovine Serum Test Material: 1′′ × 1′′ Glass Surfaces 1 mm thick non-frosted microscope slides, cut into squares Test Substance 50 μL applied and spread end to end with a pipette tip Application: Number of Wears 12 Total (6 dry and 6 wet) Performed: Number of Re-inoculations 5 Total Performed: Neutralizer Medium: 30 mL Dey Engley Broth A neutralizer screen was performed as part of the testing, verified that the neutralizer adequately neutralized the product and was not detrimental to the tested organisms. .. Plating Medium: Tryptone Glucose Extract Agar Incubation: 35° C. for 48 hours Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL MRSA Initial 148 × 103 155 × 103 1.5 × 105 ATCC 33592 Reinoculation 61 × 103 45 × 103 5.3 × 104 Final 124 × 106 134 × 106 1.3 × 108 VRE Initial 34 × 104 35 × 104 3.5 × 105 ATCC 51299 Reinoculation 94 × 103 136 × 103 1.2 × 105 Final 50 × 107 35 × 107 4.2 × 108 MRSA ATCC 33592 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 253 × 102, 263 × 102 7.59 × 105, 7.89 × 105 7.8 × 105 N/A 268 × 102, 251 × 102 8.04 × 105, 7.53 × 105 Test 0 × 100, 0 × 100 <30, <30, <30 >99.996 Composition C 0 × 100, 1 × 100 <30, 3.00 × 101 *CFU/mL × 30 VRE ATCC 51299 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 67 × 103, 90 × 103 2.01 × 106, 2.70 × 106 2.2 × 106 N/A 67 × 103, 66 × 103 2.01 × 106, 1.98 × 106 Test 4 × 102, 4 × 102 1.20 × 104, 1.20 × 104 <6.0 × 103 >99.727 Composition C 0 × 100, 0 × 100 <30, <30 *CFU/mL × 30 Test Date: Jan. 22, 2009 Date Results Read: Jan. 26, 2009 Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL Staphylococcus aureus Initial 114 × 103 100 × 103 1.1 × 105 ATCC 6538 Reinoculation 43 × 103 55 × 103 4.9 × 104 Final 111 × 106 112 × 106 1.1 × 108 Klebsiella pneumoniae Initial 117 × 103 142 × 103 1.3 × 105 ATCC 4352 Reinoculation 55 × 103 60 × 103 5.8 × 104 Final 109 × 107 129 × 107 1.2 × 109 Staphylococcus aureus ATCC 6538 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 30 × 103, 19 × 103 9.00 × 105, 5.70 × 105 8.0 × 105 N/A 31 × 103, 26 × 103 9.30 × 105, 7.80 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.996 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Klebsiella pneumoniae ATCC 4352 Survivors Survivors Average Percent Test Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 22 × 103, 18 × 103 6.60 × 105, 5.40 × 105 3.9 × 105 N/A 48 × 102, 75 × 102 1.44 × 105, 2.25 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.992 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Conclusion: To be defined as a sanitizer, the test substances on the hard inanimate surface must reduce the total number of organisms by at least 99.9% on the surface within a 5 minute period (after the final inoculation). ..
Article Title: Wear resistant antimicrobial compositions and methods of use
Article Snippet: Method Parameters: Test Substance Formula: Test composition C Raw Material Concentration Bardac DM50 (Alkyl benzyl quat 50% active)) 50% Vantocil P (PHMG 20% active) 50% Test Substance Dilutions: Prepared on Jan. 20, 2009: Desired Test Solution (Volume of Test Concentration Diluent Substance/Total Volume) 1:64 410 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.65) 1:64 510 ppm Synthetic 1.56 mL/100 mL Hard Water (pH 7.84) Test Systems: Staphylococcus aureus ATCC 6538 Klebsiella pneumoniae ATCC 4352 Staphylococcus aureus (MRSA) ATCC 33592 Enterococcus faecalis (VRE) ATCC 51299 Soil Challenge: 5% Bovine Serum Test Material: 1′′×1′′ Glass Surfaces 1 mm thick non-frosted microscope slides, cut into squares Test Substance Application: 50 μL applied and spread end to end with a pipette tip Number of Wears Performed: 12 Total (6 dry and 6 wet) Number of Re-inoculations Performed: 5 Total Neutralizer Medium: 30 mL Dey Engley Broth A neutralizer screen was performed as part of the testing, verified that the neutralizer adequately neutralized the product and was not detrimental to the tested organisms. .. Plating Medium: Tryptone Glucose Extract Agar Incubation: 35° C. for 48 hours Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL MRSA Initial 148 × 103 155 × 103 1.5 × 105 ATCC 33592 Reinoculation 61 × 103 45 × 103 5.3 × 104 Final 124 × 106 134 × 106 1.3 × 108 VRE Initial 34 × 104 35 × 104 3.5 × 105 ATCC 51299 Reinoculation 94 × 103 136 × 103 1.2 × 105 Final 50 × 107 35 × 107 4.2 × 108 MRSA ATCC 33592 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 253 × 102, 263 × 102 7.59 × 105, 7.89 × 105 7.8 × 105 N/A 268 × 102, 251 × 102 8.04 × 105, 7.53 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 >99.996 Composition C 0 × 100, 1 × 100 3.00 × 101 *CFU/mL × 30 VRE ATCC 51299 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 67 × 103, 90 × 103 2.01 × 106, 2.70 × 106 2.2 × 106 N/A 67 × 103, 66 × 103 2.01 × 106, 1.98 × 106 Test 4 × 102, 4 × 102 1.20 × 104, 1.20 × 104 <6.0 × 103 >99.727 Composition C 0 × 100, 0 × 100 <30, <30 *CFU/mL × 30 Test Date: Jan. 22, 2009 Date Results Read: Jan. 26, 2009 Inoculum Numbers Inoculum Average Test System Suspension A B CFU/mL Staphylococcus Initial 114 × 103 100 × 103 1.1 × 105 aureus Reinoculation 43 × 103 55 × 103 4.9 × 104 ATCC 6538 Final 111 × 106 112 × 106 1.1 × 108 Klebsiella Initial 117 × 103 142 × 103 1.3 × 105 pneumonias Reinoculation 55 × 103 60 × 103 5.8 × 104 ATCC 4352 Final 109 × 107 129 × 107 1.2 × 109 Staphylococcus aureus ATCC 6538 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 30 × 103, 19 × 103 9.00 × 105, 5.70 × 105 8.0 × 105 N/A 31 × 103, 26 × 103 9.30 × 105, 7.80 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.996 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Klebsiella pneumoniae ATCC 4352 Test Survivors Survivors Average Percent Substance (CFU/mL) (CFU/Carrier)* CFU/Carrier Reduction TritonX 22 × 103, 18 × 103 6.60 × 105, 5.40 × 105 3.9 × 105 N/A 48 × 102, 75 × 102 1.44 × 105, 2.25 × 105 Test 0 × 100, 0 × 100 <30, <30, <30, <30 <30 >99.992 Composition C 0 × 100, 0 × 100 *CFU/mL × 30 Conclusion: To be defined as a sanitizer, the test substances on the hard inanimate surface must reduce the total number of organisms by at least 99.9% on the surface within a 5 minute period (after the final inoculation). ..
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